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肉桂提取物对食品中AGEs的抑制作用研究

时间:2020-04-07 09:39来源:毕业论文
利用荧光检测法测定总AGEs含量,再通过SDS-聚丙烯酰胺凝胶电泳法和western blot(蛋白印迹法)进一步分析。结果表明肉桂提取物对AGEs具有良好的抑制效果

摘要晚期糖基化终产物(AGEs)在人体内的蓄积是引起许多慢性疾病如动脉粥样硬化、糖尿病等的重要原因之一。肉桂被发现具有显著的抗氧化活性,研究证明肉桂提取物含有丰富的多酚物质,因此肉桂提取物具有预防心血管疾病、增强人体免疫力和抗癌等作用。本实验拟在食品加工体系中,通过添加天然肉桂提取物来降低食品内的AGEs含量。建立牛血清蛋白(BSA)-葡萄糖的AGEs模拟生成体系,利用荧光检测法测定总AGEs含量,再通过SDS-聚丙烯酰胺凝胶电泳法和western blot(蛋白印迹法)进一步分析。结果表明肉桂提取物对AGEs具有良好的抑制效果。47500

    Accumulation of advanced glycation end products (AGEs)in vivo is associated with atherosclerosis ,diabetes, etc. Cinnamon has been found to have significant anti-oxidant activity. The study prove that cinnamon extract containing a large number of phlorotannins. Therefore, cinnamon bark extract have the prevention of cardiovascular disease, enhance immunity and anti-cancer effects. This study aimed at food processing system, by adding natural cinnamon extract to reduce AGEs content of food within. Establish bovine serum albumin (BSA) - glucose analog AGEs generation system, determination of total AGEs content using fluorescence detection method, then by SDS- polyacrylamide gel electrophoresis and western blot (Western blot) for further analysis. The results showed that cinnamon extract have good inhibitory effect on AGEs. 

毕业论文关键词:肉桂提取物;晚期糖基化终产物(AGEs);多酚物质;抑制作用

Keyword: Cinnamon bark extract; Advanced glycation end products (AGEs) ; Phlorotannins; Inhibition

目    录

引言·······················································5

1. 试验材料和仪器·············································6

1.1试验材料················································6

1.2试验试剂················································6

1.3试验仪器················································6

2. 试验方法 ··················································6 肉桂提取物对食品中AGEs的抑制作用研究:http://www.youerw.com/shiping/lunwen_49762.html

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