摘要为寻找到更多无毒、高效的天然防腐剂，以浙麦冬为研究对象，通过其对七种常见食品腐败细菌的抑菌试验，证明麦冬乙醇提取物对变形杆菌、枯草芽孢杆菌无抑制作用，但对于腐生葡萄球菌、藤黄微球菌、产气肠杆菌有显著的抑制作用，最小抑菌浓度均为0.025g/ml，且对溶酪大球菌、白色念珠菌有一定的抑制作用，二者的最小抑菌浓度均大于0.1g/ml。说明了浙麦冬开发为新型天然防腐剂的可行性。44041

Abstract To find more natural food antiseptic which are nontoxic and efficient, the antibacterial test choose the Zhejiang Radix Ophiopogonis and seven common strains of food spoilage microorganisms as research subject, prove that the aqueous solution of ethanolic extract form the Zhejiang Radix Ophiopogonis has not inhibition on Proteusbacillus vulgaris, Bacillus subtilis, but it has obvious inhibition on Staphylococcus saprophyticus, Micrococcus luteus, Enterobacter aerogenes, there minimum inhibitory concentration are all equal 0.025g/ml, and it also has helped of the inhibition on Macrococcus caseolyticus, Monilia albican,the minimum inhibitory concentration are both more than 0.1g/ml.The research shows that developing the Zhejiang Radix Ophiopogonis into natural food antiseptic is feasible.

毕业论文关键词：浙麦冬； 食品腐败细菌； 抑菌性能

Keyword: Zhejiang Radix Ophiopogonis; food spoilage microorganisms; the detection of antibacterial activity

目    录

1 选题背景与意义 5

1.1 药材的选择 5

1.2 菌种的选择 6

2 材料与方法 7

2.1实验材料、试剂与仪器 7

2.1.1 药材及菌种 7

2.1.2 试剂 7

2.1.3 仪器 7

2.2 试验方法 7

2.2.1 麦冬的粗提取 7

2.2.1.1 麦冬药材粉末的制备 7

2.2.1.2 麦冬乙醇提取物的制备 7

2.2.2 麦冬乙醇提取物抑菌能力的筛选 7

2.2.2.1 培养基的制备 7

2.2.2.2 供试菌株的恢复培养 8

2.2.2.3 菌悬液的制备 8

2.2.2.4 抑菌能力的筛选 8

2.2.3 抑菌浓度的检测 8

2.2.3.1 不同浓度梯度培养基的配制 8

2.2.3.2 对照组 9

2.2.3.3 实验组 9

2.2.3.4 最小抑菌浓度的计算 9

3 结果与分析 9

3.1 麦冬乙醇提取物抑菌能力筛选结果 9

3.2 各菌种抑菌浓度的检测 11

3.2.1 腐生葡萄球菌抑菌浓度检测 11

3.2.2 藤黄微球菌最小抑菌浓度检测 11

3.2.3 产气肠杆菌最小抑菌浓度检测 12

3.2.4 溶酪大球菌最小抑菌浓度检测 12

3.2.5 白色念珠菌最小抑菌浓度检测 13

4 讨论 13

1 选题背景和意义 麦冬乙醇提取物对七种常见食品腐败菌的抑菌性能检测:http://www.youerw.com/shiping/lunwen_45195.html