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紫甘薯花青素抑制间充质干细胞衰老的研究

时间:2020-06-12 21:35来源:毕业论文
H2O2可以降低LC3的聚集分布,表明自噬被抑制;而紫甘薯花青素可以增强LC3的点状聚集,即诱导细胞自噬。这一结果证实,紫甘薯花青素可以通过促进细胞的自噬来抑制间充质干细胞的衰

摘要间充质干细胞(bone marrow stromal cells, BMSCs)是一类有多向分化潜能和极高医学价值的细胞。但随着细胞的衰老,其分化能力受到影响,临床治疗效果不理想,因此,急需找到抑制间充质干细胞衰老的方法。研究表明 ,紫甘薯花青素具有明显的抗氧化活性,但其在间充质干细胞老化中的作用尚不明确。本实验利用H2O2预处理诱导BMSCs老化,经不同浓度的紫甘薯花青素处理后,发现紫甘薯花青素可以抑制氧化压力引起的BMSCs老化;鉴于细胞自噬与老化之间的关联,我们又检测了自噬相关指标LC3的聚集情况。结果显示,H2O2可以降低LC3的聚集分布,表明自噬被抑制;而紫甘薯花青素可以增强LC3的点状聚集,即诱导细胞自噬。这一结果证实,紫甘薯花青素可以通过促进细胞的自噬来抑制间充质干细胞的衰老。50706

该论文有图7幅,参考文献12篇。

毕业论文关键词:间充质干细胞  紫甘薯花青素  氧化压力  细胞衰老  细胞自噬

Inhibitory Impact of Purple Sweet Potato Color on Bone Marrow Stromal Cells Senescence

Abstract

Bone marrow stromal cells (BMSCs) are kinds of cells with pluripotent differentiation and high medical value. However, their differentiation ability is impaired during cell senescence, which limits the application in clinic. Therefore, it is urgent to find methods to inhibit BMSCs senescence. Papers have demonstrated the antioxidation of purple sweet potato color (PSPC), but little is known about its function in BMSCs senescence. To answer this question, BMSCs were pre-incubated with H2O2 and then administered with PSPC. Senescence-associated β-gal staining showed that PSPC could delay BMSCs senescence induced by oxidative stress and this effect was dose-dependent. Based on the relation between autophagy and senescence, we further detected the changes of LC3 puncta. The results showed that H2O2 decreased the numbers of puncta, indicating autophagy was inhibited by H2O2; On the contrary, PSPC promoted the patched distribution of LC3, suggesting PSPC could induce cell autophagy. In conclusion, our data indicated that PSPC could inhibit the senescence of BMSCs by promoting cell autophagy.

Key Words: bone marrow stromal cells  Purple sweet potato color  oxidative stress   cell senescence  cell autophagy.

目  录

摘要Ⅰ

Abstract-Ⅱ

目录Ⅲ

图清单-Ⅳ

1 绪论1

1.1 间充质干细胞的作用1

1.2 间充质干细胞存在的缺点1

1.3自噬与老化-1

1.4 紫甘薯花青素的特点2

2 材料与方法-3

2.1 实验材料3

2.2 仪器及器材-3

2.3 主要溶液及试剂配方-4

2.4 实验方法6

3 实验结果及分析-8

3.1 过氧化氢引起BMSCs8

3.2 PSPC可以抑制过氧化氢引起的BMSCs老化-8

3.3 过氧化氢抑制BMSCs的自噬9

3.4 PSPC可以促进BMSCs的自噬,进而抑制其老化-11

4 结论-12

参考文献13

致谢14

 图清单

图序号 图名称 页码

图1 β-Gal染色显示过氧化氢可以诱导BMSCs老化 8

图2 β-Gal染色显示细胞老化程度及老化细胞数目 9

图3 老化细胞比例的定量 9

图4 细胞免疫荧光显示LC3(绿色)的分布情况 10

图5 对图4中LC3阳性细胞比例的定量 紫甘薯花青素抑制间充质干细胞衰老的研究:http://www.youerw.com/yixue/lunwen_54079.html

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